Generating Diversified ssDNA Libraries of Oligonucleotides, Antibody, Peptide & Nanobody Discovery

Generating libraries of diversified oligonucleotides, especially those with sequences containing randomised codon regions, is a critical step in the development of antibody, peptide, and nanobody libraries. These libraries are foundational tools in therapeutic discovery, enabling the identification of molecules with high specificity and affinity for a wide range of targets. The methods or technologies used for generating these libraries have evolved to allow high levels of diversity, specificity, and efficiency. This podcast episode covers each method and technique in detail weighing up the advantages and disadvantages of each and considerations for their effective use.